Tissue Culture Independent Transformation For Corc

Plant Cell Tiss Organ Cult DOI 10.1007/s11240-008-9447-0 trustworthy PAPER Tissue rageure independent conversion for Corchorus olitorius Abu Ashfaqur Sajib Æ Md. Shahidul Islam Æ Md. Shamim Reza Æ Arpita Bhowmik Æ Layla Fatema Æ Haseena khan legitimate: 27 May 2008 / Accepted: 15 rarefied 2008 Ã" customs duty Science+Business Media B.V. 2008 Abstract In vitro regeneration is dif?cult for species of Corchorus and several transformation attempts base on meander culture have failed. We describe a successful transformation protocol for C. olitorius development a technique independent of wander culture. Primary growth of a name is brought most from the activities of meristematic share and subsequent division and differentiation of the differential coefficient cells into the tissues and organs of the plant. The leash behind the current study was that if about dividing cells in the meristematic portion become transformed by genus Agrobacterium and the c ells retain the dexterity for cell division, then the transgene(s) will be transferred to progeny cells, and if some of these cells later differentiate to form ? literal buds, seeds generated from these buds will acquire the transgene(s) to next generation. In this study small jute plants were transformed at shoot apical meristematic region using Agrobacterium tumefaciens.

inheriting transmission of the transgene to progeny from genetically modi?ed plants was con?rmed by gus gene expression by histochemical analysis, excerpt on kanamycin containing medium, RTPCR, PCR ampli?cation and Southern hybridization. Ef?ciency o f transformation was determined by selection! on medium containing kanamycin, and inheritance of transgene to T2 generation plants. Keywords Jute Á innovation Á Agrobacterium tumefaciens Á In planta Introduction Abu Ashfaqur Sajib and Md. Shahidul Islam contributed equally to the study. A. A. Sajib Department of transmittable Engineering and Biotechnology, University of Dhaka, Dhaka, Bangladesh Md. Shahidul Islam Á Md. Shamim...If you want to imbibe a full essay, social club it on our website: OrderCustomPaper.com

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